Western blot analysis of extracts of various cell lines, using GCLM antibody (A11444) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
GCLM Rabbit mAb
Catalog No:AB-A11444
Manufacturer:ABclonal
Synonyms:GLCLR
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | GCLM Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015] |
Immunogen: | A synthesized peptide derived from human GCLM |
Gene ID: | 2730 |
Swiss prot: | P48507 |
Molecular weight: | 31kDa |
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