Western blot analysis of extracts of Rat kidney, using GCLC Rabbit mAb (A4499) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using GCLC Rabbit mAb (A4499) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
GCLC Rabbit mAb
Catalog No:AB-A4499
Manufacturer:ABclonal
Synonyms:GCL, GCS, GLCL, GLCLC
Applications:WB,IHC
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | GCLC Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010] |
Immunogen: | A synthesized peptide derived from human GCLC |
Gene ID: | 2729 |
Swiss prot: | P48506 |
Molecular weight: | 73kDa |
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