Western blot analysis of extracts of various cell lines, using GAPDHS antibody (A10471) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
GAPDHS Rabbit pAb
Catalog No:AB-A10471
Manufacturer:ABclonal
Synonyms:GAPDHS;GAPD2;GAPDH-2;GAPDS;HEL-S-278;HSD-35
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GAPDHS Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 179-408 of human GAPDHS (NP_055179.1). |
Gene ID: | 26330 |
Swiss prot: | O14556 |
Molecular weight: | 44kDa |
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