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Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded Human esophageal using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
GAPDH Mouse mAb (High Dilution)
Catalog No：AB-AC033
Manufacturer：ABclonal
Synonyms：G3PD;GAPD;HEL-S-162eP;GAPDH
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Mouse
Conjugate：No
€65,00 – €155,00
| Product Name: | GAPDH Mouse mAb (High Dilution) |
| Category: | Tag and Loading Control Antibodies |
| Description: | This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. Note锛欴ilution method Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10,000 for using. The diluted antibody can be stored at -20鈩 without aliquot. |
| Immunogen: | Recombinant protein of human GAPDH |
| Gene ID: | 2597 |
| Swiss prot: | P04406 |
| Molecular weight: | 31kDa/36kDa |
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