Western blot analysis of extracts of various cell lines, using GAD67/GAD1 Rabbit mAb (A1475) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using GAD67/GAD1 Rabbit mAb (A1475) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
GAD67/GAD1 Rabbit mAb
Catalog No:AB-A1475
Manufacturer:ABclonal
Synonyms:CPSQ1, GAD, SCP
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | GAD67/GAD1 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human GAD67/GAD1 |
Gene ID: | 2571 |
Swiss prot: | Q99259 |
Molecular weight: | 67kDa |
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