Western blot analysis of extracts of various cell lines, using GABA transaminase (GABA transaminase (ABAT)) Polyclonal Antibody (A5299) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
Western blot analysis of extracts of HepG2 cells, using GABA transaminase (GABA transaminase (ABAT)) Polyclonal Antibody (A5299) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
GABA transaminase (ABAT) Rabbit pAb
Catalog No:AB-A5299
Manufacturer:ABclonal
Synonyms:ABAT;GABA-AT;GABAT;NPD009
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | GABA transaminase (ABAT) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-300 of human GABA transaminase (GABA transaminase (ABAT)) (NP_001120920.1). |
Gene ID: | 18 |
Swiss prot: | P80404 |
Molecular weight: | 56kDa |
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