Western blot analysis of extracts of various cell lines, using FTO antibody (A1438) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of Rat brain, using FTO antibody (A1438) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
FTO Rabbit pAb
Catalog No:AB-A1438
Manufacturer:ABclonal
Synonyms:FTO;ALKBH9;BMIQ14;GDFD
Applications:WB,IHC,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | FTO Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 416-505 of human FTO (NP_001073901.1). |
Gene ID: | 79068 |
Swiss prot: | Q9C0B1 |
Molecular weight: | 6kDa/12kDa/14kDa/58kDa |
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