Western blot analysis of extracts of various cell lines, using FAK pAb (A11131) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of MCF7 cells, using FAK pAb (A11131) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
FAK Rabbit mAb
Catalog No:AB-A11131
Manufacturer:ABclonal
Synonyms:FADK;FAK;FAK1;FRNK;PPP1R71;p125FAK;pp125FAK;PTK2
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €540,00
Product Name: | FAK Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant protein of human FAK |
Gene ID: | 5747 |
Swiss prot: | Q05397 |
Molecular weight: | 39kDa/48kDa/63kDa/99kDa/114kDa/119kDa/120kDa |
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