Western blot analysis of extracts of various cell lines, using F7 antibody (A16044) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
F7 Rabbit pAb
Catalog No:AB-A16044
Manufacturer:ABclonal
Synonyms:F7;SPCA
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | F7 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-400 of human F7 (NP_062562.1). |
Gene ID: | 2155 |
Swiss prot: | P08709 |
Molecular weight: | 49kDa/51kDa |
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