Western blot analysis of extracts of various cell lines, using EYA3 antibody (A15056) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
EYA3 Rabbit pAb
Catalog No:AB-A15056
Manufacturer:ABclonal
Synonyms:EYA3
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | EYA3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3′ UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human EYA3 (NP_001981.2). |
Gene ID: | 2140 |
Swiss prot: | Q99504 |
Molecular weight: | 48kDa/56kDa/57kDa/58kDa/62kDa |
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