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Western blot analysis of extracts of MCF7 cells, using Estrogen Receptor alpha (ESRα) antibody ( A12976) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
ESRα Rabbit mAb
Catalog No：AB-A12976
Manufacturer：ABclonal
Synonyms：ESR1;ER;ESR;ESRA;ESTRR;Era;NR3A1;ER alpha
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €540,00
| Product Name: | ESRα Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. |
| Immunogen: | A synthetic peptide of human Estrogen Receptor alpha (ESRα) |
| Gene ID: | 2099 |
| Swiss prot: | P03372 |
| Molecular weight: | 35kDa/47kDa/53kDa/66kDa |
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