Western blot analysis of extracts of various cell lines, using EIF4G3 antibody (A14621) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
EIF4G3 Rabbit pAb
Catalog No:AB-A14621
Manufacturer:ABclonal
Synonyms:EIF4G3;eIF-4G 3;eIF4G 3;eIF4GII;eIF-4G3;eIF4G3
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | EIF4G3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_003751.2). |
Gene ID: | 8672 |
Swiss prot: | O43432 |
Molecular weight: | 55kDa/146kDa/176kDa/177kDa |
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