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Western blot analysis of extracts of various cell lines, using eIF4E antibody (A6085) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 10s.
eIF4E Rabbit pAb
Catalog No：AB-A6085
Manufacturer：ABclonal
Synonyms：AUTS19;CBP;EIF4E1;EIF4EL1;EIF4F;eIF-4E;EIF4E
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | eIF4E Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5′ end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5′-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4E (NP_001959.1). |
| Gene ID: | 1977 |
| Swiss prot: | P06730 |
| Molecular weight: | 25kDa/27kDa/28kDa |
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