Western blot analysis of extracts of various cell lines, using eIF2α antibody (A0764) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
eIF2α Rabbit pAb
Catalog No:AB-A0764
Manufacturer:ABclonal
Synonyms:EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | eIF2α Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human eIF2α (NP_004085.1). |
Gene ID: | 1965 |
Swiss prot: | P05198 |
Molecular weight: | 36kDa |
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