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Western blot analysis of extracts of various cell lines, using E-Cadherin antibody (A11509) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
E-Cadherin Rabbit pAb
Catalog No：AB-A11509
Manufacturer：ABclonal
Synonyms：Arc-1;CD324;CDHE;ECAD;LCAM;UVO;CDH1;E-Cadherin
Applications：WB
Reactivity：Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | E-Cadherin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 700-800 of human E-Cadherin (NP_004351.1). |
| Gene ID: | 999 |
| Swiss prot: | P12830 |
| Molecular weight: | 90kDa/97kDa |
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