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Western blot analysis of extracts of various cell lines, using E-Cadherin antibody (A18135) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
E-Cadherin Mouse mAb
Catalog No：AB-A18135
Manufacturer：ABclonal
Synonyms：Arc-1;CD324;CDHE;ECAD;LCAM;UVO;CDH1;E-Cadherin
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Mouse
Conjugate：No
€65,00 – €540,00
| Product Name: | E-Cadherin Mouse mAb |
| Category: | Monoclonal Antibodies |
| Description: | This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. |
| Immunogen: | Recombinant protein of human E-Cadherin. |
| Gene ID: | 999 |
| Swiss prot: | P12830 |
| Molecular weight: | 90kDa/97kDa |
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