Western blot analysis of extracts of various cell lines, using DOK5 antibody (A13726) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
DOK5 Rabbit pAb
Catalog No:AB-A13726
Manufacturer:ABclonal
Synonyms:DOK5;C20orf180;IRS-6;IRS6
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DOK5 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 237-306 of human DOK5 (NP_060901.2). |
Gene ID: | 55816 |
Swiss prot: | Q9P104 |
Molecular weight: | 22kDa/35kDa |
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