Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) antibody (A16440) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 300s.
DNA topoisomerase II alpha (TOP2A) Rabbit pAb
Catalog No:AB-A16440
Manufacturer:ABclonal
Synonyms:TOP2A;TOP2;TP2A
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DNA topoisomerase II alpha (TOP2A) Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) (NP_001058.2). |
Gene ID: | 7153 |
Swiss prot: | P11388 |
Molecular weight: | 174kDa/177kDa/178kDa/182kDa |
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