Western blot analysis of extracts of various cell lines, using DNA topoisomerase I (DNA topoisomerase I (TOP1)) Rabbit mAb (A12409) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of Mouse thymus, using DNA topoisomerase I (DNA topoisomerase I (TOP1)) Rabbit mAb (A12409) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
DNA topoisomerase I (TOP1) Rabbit mAb
Catalog No:AB-A12409
Manufacturer:ABclonal
Synonyms:TOPI
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | DNA topoisomerase I (TOP1) Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
Immunogen: | A synthesized peptide derived from human DNA topoisomerase I (DNA topoisomerase I (TOP1)) |
Gene ID: | 7150 |
Swiss prot: | P11387 |
Molecular weight: | 91kDa |
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