Western blot analysis of extracts of various cell lines, using DISP1 antibody (A14946) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
DISP1 Rabbit pAb
Catalog No:AB-A14946
Manufacturer:ABclonal
Synonyms:DISP1;DISPA
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DISP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structures often depends upon the localized production of secreted protein signals. Cells surrounding the source of a particular signal respond in a graded manner according to the effective concentration of the signal, and this response produces the pattern of cell types constituting the mature structure. A novel segment-polarity gene known as dispatched has been identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is one of two human homologs of Drosophila dispatched and, based on sequence identity to its mouse counterpart, the encoded protein may play an essential role in Hh patterning activities in the early embryo. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1325-1524 of human DISP1 (NP_116279.2). |
Gene ID: | 84976 |
Swiss prot: | Q96F81 |
Molecular weight: | 170kDa |
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