Western blot analysis of extracts of various cell lines, using DGKE antibody (A7752) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
DGKE Rabbit pAb
Catalog No:AB-A7752
Manufacturer:ABclonal
Synonyms:DGKE;AHUS7;DAGK5;DAGK6;DGK;NPHS7
Applications:WB,IHC,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DGKE Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human DGKE (NP_003638.1). |
Gene ID: | 8526 |
Swiss prot: | P52429 |
Molecular weight: | 20kDa/63kDa |
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