Western blot analysis of extracts of various cell lines, using DGKB antibody (A10534) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
DGKB Rabbit pAb
Catalog No:AB-A10534
Manufacturer:ABclonal
Synonyms:DGKB;DAGK2;DGK;DGK-BETA
Applications:WB
Reactivity:Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DGKB Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 544-773 of human DGKB (NP_663733.1). |
Gene ID: | 1607 |
Swiss prot: | Q9Y6T7 |
Molecular weight: | 87kDa/90kDa |
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