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Western blot analysis of extracts of various cell lines, using DCX antibody (A1134) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
DCX Rabbit pAb
Catalog No：AB-A1134
Manufacturer：ABclonal
Synonyms：DBCN;DC;LISX;SCLH;XLIS;DCX
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | DCX Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia (‘double cortex’ syndrome) in females and lissencephaly (‘smooth brain’ syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 92-441 of human DCX (NP_000546.2). |
| Gene ID: | 1641 |
| Swiss prot: | O43602 |
| Molecular weight: | 40kDa |
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