Western blot analysis of extracts of various cell lines, using DAZ1 antibody (A5310) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
DAZ1 Rabbit pAb
Catalog No:AB-A5310
Manufacturer:ABclonal
Synonyms:DAZ1;DAZ;SPGY
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | DAZ1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 340-530 of human DAZ1 (NP_004072.3). |
Gene ID: | 1617 |
Swiss prot: | Q9NQZ3 |
Molecular weight: | 67kDa/82kDa |
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