.jpg)
Western blot analysis of extracts of various cell lines, using Cytochrome C antibody (A0225) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Cytochrome C antibody (A0225) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunofluorescence analysis of NIH/3T3 cells using Cytochrome C Rabbit pAb (A0225) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts from normal (control) and Cytochrome C knockout (KO) 293T cells, using Cytochrome C antibody (A0225) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Cytochrome C Rabbit pAb
Catalog No：AB-A0225
Manufacturer：ABclonal
Synonyms：CYCS;CYC;HCS;THC4;cytochrome c
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Cytochrome C Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-105 of human Cytochrome C (NP_061820.1). |
| Gene ID: | 54205 |
| Swiss prot: | P99999 |
| Molecular weight: | 11kDa |
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