Western blot analysis of extracts of various cell lines, using CYP7A1 antibody (A10615) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
CYP7A1 Rabbit pAb
Catalog No:AB-A10615
Manufacturer:ABclonal
Synonyms:CYP7A1;CP7A;CYP7;CYPVII
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CYP7A1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-280 of human CYP7A1 (NP_000771.2). |
Gene ID: | 1581 |
Swiss prot: | P22680 |
Molecular weight: | 57kDa |
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