Western blot analysis of extracts of various cell lines, using Cyclin E2 antibody (A4272) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Cyclin E2 Rabbit pAb
Catalog No:AB-A4272
Manufacturer:ABclonal
Synonyms:CCNE2;CYCE2;cyclin E2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cyclin E2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human Cyclin E2 (NP_477097.1). |
Gene ID: | 9134 |
Swiss prot: | O96020 |
Molecular weight: | 41kDa/46kDa |
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