Western blot analysis of extracts of rat liver, using Cyclin D3 antibody (A1084) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Cyclin D3 Rabbit pAb
Catalog No:AB-A1084
Manufacturer:ABclonal
Synonyms:CCND3;cyclin D3;Cyclin D3
Applications:WB
Reactivity:Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cyclin D3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human Cyclin D3 (NP_001751.1). |
Gene ID: | 896 |
Swiss prot: | P30281 |
Molecular weight: | 9kDa/22kDa/24kDa/32kDa |
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