Western blot analysis of extracts of various cell lines, using Cullin 3 antibody (A1078) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
Cullin 3 Rabbit pAb
Catalog No:AB-A1078
Manufacturer:ABclonal
Synonyms:CUL-3;PHA2E;CUL3;Cullin 3;cullin-3
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Cullin 3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 569-768 of human Cullin 3 (NP_003581.1). |
Gene ID: | 8452 |
Swiss prot: | Q13618 |
Molecular weight: | 81kDa/86kDa/88kDa |
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