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Western blot analysis of extracts of various cell lines, using CTSF antibody (A10469) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human breast cancer using CTSF antibody (A10469) at dilution of 1:100 (40x lens).
CTSF Rabbit pAb
Catalog No：AB-A10469
Manufacturer：ABclonal
Synonyms：CTSF;CATSF;CLN13
Applications：WB,IHC,IF
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CTSF Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 270-484 of human CTSF (NP_003784.2). |
| Gene ID: | 8722 |
| Swiss prot: | Q9UBX1 |
| Molecular weight: | 53kDa |
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