Western blot analysis of extracts of various cell lines, using CPT1A antibody (A5307) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
CPT1A Rabbit pAb
Catalog No:AB-A5307
Manufacturer:ABclonal
Synonyms:CPT1A;CPT1;CPT1-L;L-CPT1
Applications:WB,IP
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CPT1A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 497-756 of human CPT1A (NP_001027017.1). |
Gene ID: | 1374 |
Swiss prot: | P50416 |
Molecular weight: | 86kDa/88kDa |
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