Western blot analysis of extracts of various cell lines, using COX5A Antibody (A6437) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
COX5A Rabbit pAb
Catalog No:AB-A6437
Manufacturer:ABclonal
Synonyms:COX5A;COX;COX-VA;VA
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | COX5A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human COX5A (NP_004246.2). |
Gene ID: | 9377 |
Swiss prot: | P20674 |
Molecular weight: | 16kDa |
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