Western blot analysis of extracts of various cell lines, using Cox2 Rabbit pAb (A1253) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Cox2 Rabbit pAb
Catalog No:AB-A1253
Manufacturer:ABclonal
Synonyms:PTGS2;COX-2;COX2;GRIPGHS;PGG/HS;PGHS-2;PHS-2;hCox-2;Cox2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €1.760,00
Product Name: | Cox2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human Cox2 (NP_000954.1). |
Gene ID: | 5743 |
Swiss prot: | P35354 |
Molecular weight: | 68kDa |
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