Western blot analysis of extracts of various cell lines, using COX15 antibody (A14665) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
COX15 Rabbit pAb
Catalog No:AB-A14665
Manufacturer:ABclonal
Synonyms:COX15;CEMCOX2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | COX15 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates two transcript variants diverging in the 3′ region. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human COX15 (NP_510870.1). |
Gene ID: | 1355 |
Swiss prot: | Q7KZN9 |
Molecular weight: | 43kDa/46kDa |
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