Western blot analysis of extracts of various cell lines, using Complement C3 antibody (A13283) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Complement C3 Polyclonal Antibody (A13283) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Complement C3 Rabbit pAb
Catalog No:AB-A13283
Manufacturer:ABclonal
Synonyms:C3;AHUS5;ARMD9;ASP;C3a;C3b;CPAMD1;HEL-S-62p;complement C3;C3 / C3a
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Complement C3 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1364-1663 of human Complement C3 (NP_000055.2). |
Gene ID: | 718 |
Swiss prot: | P01024 |
Molecular weight: | 187kDa |
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