Western blot analysis of extracts of various cell lines, using COMP antibody (A5812) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
COMP Rabbit pAb
Catalog No:AB-A5812
Manufacturer:ABclonal
Synonyms:COMP;EDM1;EPD1;MED;PSACH;THBS5;TSP5
Applications:WB,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | COMP Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2). |
Gene ID: | 1311 |
Swiss prot: | P49747 |
Molecular weight: | 77kDa/82kDa |
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