Western blot analysis of extracts of various cell lines, using COL1A2 antibody (A5786) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
COL1A2 Rabbit pAb
Catalog No:AB-A5786
Manufacturer:ABclonal
Synonyms:COL1A2;OI4
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | COL1A2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human COL1A2 (NP_000080.2). |
Gene ID: | 1278 |
Swiss prot: | P08123 |
Molecular weight: | 129kDa |
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