Western blot analysis of extracts of various cell lines, using COASY antibody (A12179) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
COASY Rabbit pAb
Catalog No:AB-A12179
Manufacturer:ABclonal
Synonyms:COASY;DPCK;NBIA6;NBP;PPAT;UKR1;pOV-2
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | COASY Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4′-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 345-564 of human COASY (NP_079509.5). |
Gene ID: | 80347 |
Swiss prot: | Q13057 |
Molecular weight: | 62kDa/65kDa |
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