Western blot analysis of extracts of BxPC-3 cells, using Coagulation factor III/Tissue Factor antibody (A1378) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Coagulation factor III/Tissue Factor antibody (A1378) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Coagulation factor III/Tissue Factor Rabbit pAb
Catalog No:AB-A1378
Manufacturer:ABclonal
Synonyms:F3;CD142;TF;TFA
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | Coagulation factor III/Tissue Factor Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-251 of human Coagulation factor III/Tissue Factor (NP_001984.1). |
Gene ID: | 2152 |
Swiss prot: | P13726 |
Molecular weight: | 27kDa/33kDa |
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