Western blot analysis of extracts of various cell lines, using CHD4 antibody (A11574) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
CHD4 Rabbit pAb
Catalog No:AB-A11574
Manufacturer:ABclonal
Synonyms:CHD4;CHD-4;Mi-2b;Mi2-BETA;SIHIWES
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CHD4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2). |
Gene ID: | 1108 |
Swiss prot: | Q14839 |
Molecular weight: | 218kDa/220kDa |
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