Western blot analysis of extracts of various cell lines, using CFL1 antibody (A1704) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3s.
CFL1 Rabbit pAb
Catalog No:AB-A1704
Manufacturer:ABclonal
Synonyms:CFL1;CFL;HEL-S-15;cofilin;cofilin-1
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CFL1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CFL1 (NP_005498.1). |
Gene ID: | 1072 |
Swiss prot: | P23528 |
Molecular weight: | 18kDa |
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