Western blot analysis of extracts of HeLa cells, using CENPF antibody (A18644) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Western blot analysis of extracts of Mouse brain, using CENPF antibody (A18644) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
CENPF Rabbit pAb
Catalog No:AB-A18644
Manufacturer:ABclonal
Synonyms:CENF, CILD31, PRO1779, STROMS, hcp-1
Applications:WB
Reactivity:Human,Mouse,
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CENPF Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008] |
Immunogen: | Recombinant protein of human CENPF. |
Gene ID: | 1063 |
Swiss prot: | P49454 |
Molecular weight: |
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