Western blot analysis of extracts of various cell lines, using CDKN2B antibody (A5685) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
CDKN2B Rabbit pAb
Catalog No:AB-A5685
Manufacturer:ABclonal
Synonyms:CDKN2B;CDK4I;INK4B;MTS2;P15;TP15;p15INK4b
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CDKN2B Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human CDKN2B (NP_004927.2). |
Gene ID: | 1030 |
Swiss prot: | P42772 |
Molecular weight: | 8kDa/14kDa |
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