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Western blot analysis of extracts of various cell lines, using CDH5 antibody (A12416) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of HUVEC cells, using CDH5 antibody (A12416) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using CDH5 Rabbit pAb (A12416) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using CDH5 Rabbit pAb (A12416) at dilution of 1:150 (40x lens).
CDH5 Rabbit pAb
Catalog No：AB-A12416
Manufacturer：ABclonal
Synonyms：CDH5;7B4;CD144;cadherin-5
Applications：WB,IHC
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CDH5 Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 650-750 of human CDH5 (NP_001786.2). |
| Gene ID: | 1003 |
| Swiss prot: | P33151 |
| Molecular weight: | 74kDa/87kDa |
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