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Western blot analysis of extracts of A-549 cells, using CDC25B antibody (A0534) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
CDC25B Rabbit pAb
Catalog No：AB-A0534
Manufacturer：ABclonal
Synonyms：CDC25B
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | CDC25B Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CDC25B (NP_068659.1). |
| Gene ID: | 994 |
| Swiss prot: | P30305 |
| Molecular weight: | 60kDa/63kDa/64kDa/67kDa |
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