Western blot analysis of extracts of various cell lines, using CDC25A antibody (A1173) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
CDC25A Rabbit pAb
Catalog No:AB-A1173
Manufacturer:ABclonal
Synonyms:CDC25A;CDC25A2
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CDC25A Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 235-484 of human CDC25A (NP_963861.1). |
Gene ID: | 993 |
Swiss prot: | P30304 |
Molecular weight: | 54kDa/59kDa |
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