Western blot analysis of extracts of various cell lines, using CD97 antibody (A3780) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CD97 Rabbit pAb
Catalog No:AB-A3780
Manufacturer:ABclonal
Synonyms:ADGRE5;CD97;TM7LN1
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CD97 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-435 of human CD97 (NP_001775.2). |
Gene ID: | 976 |
Swiss prot: | P48960 |
Molecular weight: | 81kDa/86kDa/91kDa |
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