Western blot analysis of extracts of various cell lines, using CD74 antibody (A5667) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
CD74 Rabbit pAb
Catalog No:AB-A5667
Manufacturer:ABclonal
Synonyms:CD74;DHLAG;HLADG;II;Ia-GAMMA
Applications:WB,IF
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CD74 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 73-232 of human CD74 (NP_004346.1). |
Gene ID: | 972 |
Swiss prot: | P04233 |
Molecular weight: | 18kDa/26kDa/33kDa |
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