Western blot analysis of extracts of various cell lines, using CD4 antibody (A1549) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
CD4 Rabbit pAb
Catalog No:AB-A1549
Manufacturer:ABclonal
Synonyms:CD4;CD4mut
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CD4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD4 (NP_000607.1). |
Gene ID: | 920 |
Swiss prot: | P01730 |
Molecular weight: | 51kDa |
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