Western blot analysis of extracts of various cell lines, using CD3E antibody (A12413) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
CD3E Rabbit pAb
Catalog No:AB-A12413
Manufacturer:ABclonal
Synonyms:CD3E;IMD18;T3E;TCRE;CD3e molecule
Applications:WB,IHC
Reactivity:Mouse
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | CD3E Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human CD3E (NP_000724.1). |
Gene ID: | 916 |
Swiss prot: | P07766 |
Molecular weight: | 23kDa |
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